Analytical capabilities of coherent anti-Stokes Raman scattering microspectroscopy
نویسندگان
چکیده
منابع مشابه
Triple-resonance coherent anti-stokes Raman scattering microspectroscopy.
Fluorescence-free microscopy: A new nonlinear optical microspectroscopy technique, femtosecond (fs) triple-resonance coherent anti-Stokes Raman scattering, in which the amplitude and phase of input fs laser pulses are optimally shaped to be in triple resonance with the molecular electronic and vibrational transitions, generates a coherent nonlinear signal beam at a new color with a highest poss...
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A tutorial article is presented for the use of linear and nonlinear Raman microspectroscopies in biomedical diagnostics. Coherent anti-Stokes Raman scattering (CARS) is the most frequently applied nonlinear variant of Raman spectroscopy. The basic concepts of Raman and CARS are introduced first, and subsequent biomedical applications of Raman and CARS are described. Raman microspectroscopy is a...
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We report polarization coherent anti-Stokes Raman scattering (P-CARS) microscopy that allows vibrational imaging with high sensitivity and spectral selectivity. The nonresonant background signals from both Raman scatterers and the solvent are efficiently suppressed in P-CARS microscopy. We demonstrate P-CARS imaging of unstained cells based on the contrast of the protein amide I band.
متن کاملBroadband Coherent Anti-Stokes Raman Scattering
Broadband coherent anti-Stokes Raman scattering (BCARS) microspectroscopy is a unique label-free imaging modality that provides detailed chemical information at each pixel. In minutes, this technique captures a “hyperspectral” cube that co-registers microscopic spatial features with chemical signatures, sensitive to molecular content and structure. This technique can delve into the molecular in...
متن کاملMultiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy and Study of Lipid Vesicles
We report a theoretical description and experimental implementation of multiplex coherent anti-Stokes Raman scattering (M-CARS) microspectroscopy using a picosecond pump beam and a femtosecond Stokes beam. The effect of the chirp in the Stokes pulse on a M-CARS spectrum is studied. Polarization-sensitive detection is utilized for suppression of the nonresonant background and for selective detec...
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ژورنال
عنوان ژورنال: Journal of Modern Optics
سال: 2008
ISSN: 0950-0340,1362-3044
DOI: 10.1080/09500340802168639